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Protein Purification from Polyacrylamide Gels by Sonication Extraction Claudio A. Retamal,*,† Paula Thiebaut,* and Elias W. Alves*,‡,1 *Centro de Biocieˆncias e Biotecnologia (CBB/LQFPP), Universidade Estadual do Norte Fluminense, Rio de Janeiro, Brasil; ‡Departamento de Bioquı´mica Me´dica (DBM/ICB), Universidade Federal de Rio de Janeiro, Rio de Janeiro, Brasil; and
Scale-up from the shake-flask level to a laboratory-scale bioreactor further enhanced the enzyme yield by 0.809 UmL-1. The molecular weight of the enzyme (40 kDa), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, closely resembled the PHB depolymerase of Aureobacterium saperdae.
