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how to dissolve Polyacrylamide gel? - posted in Electrophoresis: Hi All, Does some body know how to dissolve Polyacrylamide gel? I want to segregate a lipopolysaccharide molecule (relatively DNA/RNA/ Protein Free), which I can detect on SDS PAGE using silver staining. I want to slice the gel band and hopefully dissolve poly acrylamide gel, without destroying product. are there any commercial
Western Blot is composed of polyacrylamide gel electrophoresis (PAGE), followed by an electrophoretic transfer onto a membrane (mostly PVDF or Nitrocellulose) and an immunostaining procedure to visualize a certain protein on the blot membrane. SDS-PAGE is a standard means for separating proteins according to their molecular weight.
