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The gel was run at a constant electric field of 8 V/cm; denaturing gel electrophoresis: 20% polyacrylamide, 1× TBE, 8 m urea; loading buffer: 80% formamide; 1× TBE; 0.05% bromphenol blue; 0,05% xylene cyanole. The gel was run at a constant
M urea, 2× TBE, 0.03% bromophenol blue, 0.03% xylene cyanol, 10 mM EDTA, 10 mM EGTA). The samples were heated to 95 C for 5 min, briefly centrifuged and applied to urea-PAGE (6% polyacrylamide gel containing 7 M urea in 1× TBE). The gel was
