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In this work, sequential rat glomerular isolation, ultrasonic disruption, trypsin digestion, ion-exchange chromatography, and preparative polyacrylamide gel electrophoresis have been used to isolate anionic sialoglycoproteins from the glomerular
1967) and Weber and Osborne, (1969) have shown that the separation of proteins by polyacrylamide gel electrophoresis in the presence of anionic detergents sodium dodecyl sulphate is dependent on the molecular weight of the polypeptide chains.The
