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5% SDS is a detergent surfactant commonly used in preparing proteins for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins and as an additive to transfer buffer in western blotting. SDS increases the elution rate of proteins from the gel. Reference number: 51-20-01
In SDS-PAGE, the use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel largely eliminates the influence of the structure and charge, and proteins are separated solely based on polypeptide chain length.
