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Fig. 1. Discontinuous electrophoresis of the bovine heart cytochrome-c oxidase on a gradient polyacrylamide gel under nondenaturing conditions (native DGPAGE). Separation was performed in the presence of nonionic detergent as described in
The Triton gradient protocol in the discontinuous gel system, developed by Bonner and coworkers (), is described in Section 3. It has a distinct advantage over the urea gradient protocol. Generally, it can identify a core histone protein band as
