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Prepare 10%of resolving gel and 4.5% of stacking gel. NOTE:Please refer to appendix 1 for the recipe. Prepare the separating gel solution by combining all reagents. Do not add Ammonium per sulfate and TEMED. Add APS and TEMED to the monomer
Polyacrylamide gel electrophoresis (PAGE) is a technique use almost universally in life science laboratories. The goal of this technique is to separate a mixed sample of proteins to identify and quantify single proteins from the mixture. The
