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Gradient blue native polyacrylamide gel electrophoresis (BN‐PAGE) is a well established and widely used technique for activity analysis of high‐molecular‐weight proteins, protein complexes, and protein‐protein interactions.
Takeoka T, Gotoh F, Furumi K, Mori K. Polyacrylamide-gel disc electrophoresis of native cerebrospinal fluid proteins with special reference to immunoglobulins and some clinical applications. J Neurol Sci. 1976 Oct; 29 (2-4):213–239. Maidment BW, Jr, Papsidero LD, Chu TM. Isoelectric focusing--a new approach to the study of immune complexes.
