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if you don't have labile crosslinker, as suggested by proteamatt, then you can digest polyacrylamide with the wet oxidation method: using freshly prepared 60% perchloric acid and 30% hydrogen peroxide, slice the gel into 3-5mm strips with a sharp blade. place the slices into a glass vial (we use scintillation vials), one per vial.
Polyacrylamide gel electrophoresis (PAGE) has been widely used for the analysis of glycosaminoglycans and glycosaminoglycan-derived oligosaccharides prepared by enzymatic and chemical methods. Cowman et al. first described the separation of GAGs and their oligosaccharides in a 10% polyacrylamide gel matrix visualized by alcian blue staining (21).Rice et al. introduced the use of a