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#01 Polyacrylamide Slab Gel Electrophoresis of Proteins Hassan Ahmad, University of Texas-Pan American, Edinburg, TX 78539 INTRODUCTION Description Electrophoresis involves the transport of charged molecules through a solvent in an electrical field. Many practical laboratory techniques have been developed to apply the principles of electrophoresis.
Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.Electrophoretic mobility is a function of the length, conformation and charge of the molecule.
