orange g anionic polyacrylamide gel specifications from Angola
Polyacrylamide (12.5 %) gel slab electrophoresis in 0.1% sodium dodecyl sulphate was carried out as described by Laemmli (9). Analytical IEF in the pH range 2.5–7.0 was performed on 5.0 % acrylamide gel slab using a Multiphor II electrophoresis system (Pharmacia Biotech) following the manufacturer's instructions. Non-denaturing.
This electrophoresis platform comprises horizontal and vertical gel electrophoresis units in addition to blotting systems. These systems are suitable for a variety of DNA, RNA and protein gel electrophoresis, including research applications involving nucleic acid purification, protein isolation and purification, protein expression/recombinant
