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RESULTS AND DISCUSSION In table 1 the quantities of proteins coupled to glutaraldehyde-activated polyacrylamide-agarose beads (AcA34) using various concentrations of proteins and beads are given. Under the conditions described, 0.4 to 2.0 mg of proteins per ml of polyacrylamide-agarose gel were found to be fixed.
Several enzymes have been coupled to polyacrylamide beads with glutaraldehyde, and the optimum conditions of activation and linkage have been determined by Weston and Avrameas. 1° BioGel P300 minus 400 mesh was used, and pH 6.9 was found to be the optimum pH for activation with 6% glutaraldehyde.
