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For example,while a 20 % gel can be electrophoresed at 800 V with few problems, an8 % gel under the same conditions would likely generate too much heat forthe apparatus to dissipate. 13. When the oligonucleotideis sufficiently resolved, turn off
Centrifuge at 12000 x g for 10 minutes. Use the supernatant. good Luck. Put the piece of gel on top of a Spin-X tube-filter (Corning), spin for 10 minutes and use the fluid to precipitate the DNA
