transfer and degradation of polyacrylamide-based flocculants
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Preparation of samples: Mix your protein in the ratio 4:1 with the sample buffer. Heat your sample by either: a) Boiling for 5-10 minutes. (Works for most proteins.) b) 65°C for 10 minutes. c) 37°C for 30 minutes. Running the gel: Note : Before running the gel make sure that the gel, gel apparatus and samples are ready.
