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Polyacrylamide gel electrophoresis (PAGE) has been widely used for the analysis of glycosaminoglycans and glycosaminoglycan-derived oligosaccharides prepared by enzymatic and chemical methods. Cowman et al. first described the separation of GAGs
17 TrisÐAcetate Polyacrylamide Gradient GelsÉ 207 the sample is heated at 100 C in Laemmli sample buffer ( 8 ) . After the run, proteins in the gel can be stained (e.g., CBB) or trans-ferred to
