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single-uracil templates. (a) Denaturing polyacrylamide gel showing products of reactions in which a 31-mer primer was extended against a 119-nucleotide template (see Methods) containing either a deoxyuri-dine (U) or a deoxythymidine (T) 23
were resolved on a 15% denaturing polyacrylamide gel and visualized by autoradiography. Lanes 1, 3, and 5, reaction mixtures without en- zyme; lunes 2, 4, and 6, reaction mixtures contained purified human DNase IV (0.1 pg of protein). Numbers on