uses of synthesis and application of nonionic polyacrylamide in europe
SDS-PAGE.Sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was carried out by the methodofLaemmli(24) in 12.5% polyacrylamide slabs, and proteins were stained as described by Fairbankset al. (12). Molecularweight(MW)markers
Polyacrylamide gels in the range of 4–8% are typically used, although it is not uncommon for higher percentage gels to be used with smaller probes and complexes. Agarose gels (0.7–1.2%) can be used to resolve very large complexes, as is the case
