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Before SDS-PAGE, protein samples are denatured using an ionic detergent, such as SDS, and heat. Reducing agents, such as beta-mercaptoethanol, are often used to eliminate disulfide bonds in the proteins. Then the denatured and reduced lysate is
SDS, an anionic detergent, is used to produce an even charge across the length of proteins that have been linearized. By first loading them into a gel made of polyacrylamide and then applying an electric field to the gel,…
