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SDS-PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field
The polyacrylamide gels used to separate proteins are formed by the chemical polymerization of acrylamide and a cross-linking reagent, N,N’methylenebisacrylamide (opposite page). Investigators are able to control the size of the pores in the gel
