lowest cost of orange g polyacrylamide gel from Azerbaijan

lowest cost of orange g polyacrylamide gel from Azerbaijan
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  • Does Methyl green stain agarose & polyacrylamide gels?
  • DNA staining in agarose and polyacrylamide gels by methyl green Methyl green (MG) is an inexpensive, nonproprietary, traditional histological stain for cell nuclei. When bound to DNA and upon excitation with orange-red light, it fluoresces brightly in the far red region.
  • Which acrylamide concentration should be adjusted?
  • Depending on the required resolution and size of macromolecules, the final acrylamide concentration should be adjusted accordingly. We use 8% polyacrylamide for RNAs of ~10-50 bases. Larger proteins and longer nucleic acids should be resolved on a lower percentage polyacrylamide gel. Smaller substrates may resolve better on higher percentage gels.
  • How fragile is acrylamide gel?
  • The fragility of the gel will depend on the percentage of acrylamide used. The lower the acrylamide percentage, the more fragile the gel. To minimize the chance of gel tearing, the tools and glass plates used to manipulate the gels should be kept wet by deionized water at all times.
  • How much nucleic acid moves through a polyacrylamide gel?
  • For typical gels, this is approximately 5 μl. While the nucleic acid alone will likely move through the polyacrylamide matrix, the mobility of protein (or protein-nucleic acid complex) through the gel will primarily depend on two factors. Firstly, the molecular weight (and shape) of the protein affects how the complex migrates through the gel.