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- What is polyacrylamide gel electrophoresis (PAGE)?
- To follow this article, as basic understanding of protein biochemistry is helpful. Polyacrylamide gel electrophoresis (PAGE) is a technique use almost universally in life science laboratories. The goal of this technique is to separate a mixed sample of proteins to identify and quantify single proteins from the mixture.
- What is acrylamide gel electrophoresis?
- Acrylamide Gel Electrophoresis Polyacrylamide gel electrophoresis provides very high resolution of DNA molecules 10–3,000 bp long. Under the appropriate conditions, DNA molecules differing in size by only a single base pair can be resolved (learn more: Nucleic acid electrophoresis education ).
- What type of gel is used for electrophoresis?
- Electrophoresis gels are typically agarose or polyacrylamide; each is suited to different molecules. Polyacrylamide gel electrophoresis (PAGE) is usually used for proteins and smaller DNA fragments. Polyacrylamide gels form by chemical reaction and have uniform pore sizes. They can be cast by the user or purchased as ready-to-use or pre-cast gels.
- Why is polyacrylamide better than agarose?
- The pores formed in polyacrylamide are smaller than those of agarose, used for agarose gel electrophoresis. This makes it more suitable for the separation of proteins over large polynucleotide DNA or RNA fragments and allows the separation of relatively small proteins.
