polyacrylamide bead sensors for in vivo quantification in russia

polyacrylamide bead sensors for in vivo quantification in russia
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  • Can cell-like polyacrylamide bead sensors detect cell-scale stresses?
  • Here, we describe and characterize cell-like polyacrylamide (PAAm) bead sensors with well-defined elastic properties and size for in vivo quantification of cell-scale stresses. The beads were injected into developing zebrafish embryos and their deformations were computationally analyzed to delineate spatio-temporal local acting stresses.
  • How do polyacrylamide beads measure cell-scale stress in zebrafish development?
  • Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development Mechanical stress exerted and experienced by cells during tissue morphogenesis and organ formation plays an important role in embryonic development.
  • Can pll-cy3 functionalized PAAM beads be used to measure cellular stress?
  • The present work has demonstrated that PLL-Cy3 functionalized PAAm beads can be efficiently injected into living tissue/embryos to quantitatively determine in vivo cellular stresses. For the first time, a reliable reference configuration has been proposed to numerically analyze bead deformations that deviate from the spherical shape in vivo.
  • Are polyacrylamide (PAAM) beads bioadhesive?
  • To render the inert PAAm beads bioadhesive, and to allow a visualization of the stress response in real time, they were covalently modified with Poly-L-Lysine (PLL) conjugated with Cy3 fluorophores (PLL-Cy3) via NHS-ester, after production (right inset Fig. 1a) 28. Mechanical characterization of polyacrylamide (PAAm) beads.