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- What is polyacrylamide gel electrophoresis (PAGE)?
- Preparative polyacrylamide gel electrophoresis (PAGE) is a powerful tool for purifying RNA samples. Denaturing PAGE allows separation of nucleic acids that differ by a single nucleotide in length.
- How is RNA extracted from a polyacrylamide gel?
- Overview RNA will be extracted from the gel using the ‘crush and soak’ method (see also Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)). 8.2. Duration
- What are the elution conditions for a polyacrylamide gel?
- The elution conditions can vary greatly depending on the electroelution apparatus, the acrylamide percentage of the gel, the membranes, and the buffer used. A good starting point is to conduct the electrophoresis in 1× TBE at 100V for 2h. This should be sufficient to elute ~100 nucleotide long RNA molecules from a 4% polyacrylamide gel.
- How does a polyacrylamide gel separate analytes?
- The basic principle of PAGE is to separate analytes by passing them through the pores of a polyacrylamide gel using an electric current. To achieve this, an acrylamide– bisacrylamide mix is polymerized (polyacrylamide) by the addition of ammonium persulfate (APS).
