suppliers for cationic polyacrylamide gel electrophoresis protocol

suppliers for cationic polyacrylamide gel electrophoresis protocol
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  • What is polyacrylamide gel electrophoresis (PAGE)?
  • Introduction Polyacrylamide gel electrophoresis (PAGE) is one of the most widely used methods to analyze proteins biochemically [ 1 ]. After separation by electrophoresis, protein bands need to be detected with a subsequent staining step.
  • What is acrylamide gel electrophoresis?
  • Acrylamide Gel Electrophoresis Polyacrylamide gel electrophoresis provides very high resolution of DNA molecules 10–3,000 bp long. Under the appropriate conditions, DNA molecules differing in size by only a single base pair can be resolved (learn more: Nucleic acid electrophoresis education ).
  • Why do we use polyacrylamide instead of agarose?
  • In PAGE, rather than agarose, we use a chemical called polyacrylamide. Varying the percentage of polyacrylamide in the gel lets us change the size of the pores in the gel, which means that we can separate different sizes of protein in different percentage gels. Typical gel percentages are shown in the table below.
  • What is the best stain for detecting proteins in polyacrylamide gels?
  • Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255–262. Oakley BR et al. (1980). A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105, 361–363.