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- Can fluorescent multivalent chelator probes detect protein in polyacrylamide gel electrophoresis (PAGE)?
- Fluorescent multivalent chelator probes may also offer specific and stable labeling for convenient ultrafast protein detection in polyacrylamide gel electrophoresis (PAGE). Here, we report on various in-gel fluorescence methods summarized as HisQuick-PAGE (Quick His-tag detection).
- Can hexa NTA fluorophore detect his tagged proteins in SDS-PAGE?
- Here, we report on various in-gel fluorescence methods summarized as HisQuick-PAGE (Quick His-tag detection). We elaborate that the super-chelator hexa NTA fluorophore allows nearly background-free in-gel detection of His 10 - or His 12 -tagged proteins in sodium dodecyl sulfate (SDS)-PAGE.
- Can a polyhistidine tag be used for protein purification?
- In conjunction with these methods, the polyhistidine-tag has proven to be a superb fusion tag for protein purification as well as specific protein detection by immunoblotting, which led to a vast amount of commercially available antibodies. Nevertheless, antibody batch-to-batch variations and nonspecific binding complicate the laborious procedure.
