microfluidic polyacrylamide gel electrophoresis at malaysia

microfluidic polyacrylamide gel electrophoresis at malaysia
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  • What is polyacrylamide gel electrophoresis (PAGE)?
  • Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.
  • Can a two-dimensional microfluidic architecture integrate polyacrylamide gel electrophoresis and immunoblotting?
  • We demonstrate a two-dimensional microfluidic architecture that integrates polyacrylamide gel electrophoresis (PAGE) with immunoblotting in a fully automated format.
  • Can a single streamlined microfluidic assay report protein electrophoretic mobility and identity?
  • Department of Bioengineering, University of California, Berkeley, California 94720, USA. We introduce an automated immunoblotting method that reports protein electrophoretic mobility and identity in a single streamlined microfluidic assay. Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting.
  • What is an internal standard in microfluidic electrophoresis?
  • An internal standard (IS), such as fluorescein, fluorescenceconjugated proteins or nucleic acids, is commonly used in quantitative microfluidic electrophoresis to account for chipto-chip and run-to-run assay variations [62, 63].