us3002960a – polyacrylamide preparation – chemicals patents

us3002960a – polyacrylamide preparation – chemicals patents
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  • Who invented polyacrylamide gel?
  • Raymond and Weintraub (1959) were the first to recognise the applicability of polyacrylamide gels as a supporting medium for electrophoretic separations. The synthetic polymer, polyacrylamide, is simply produced by polymerising acrylamide with N,N′-methylene-bisacrylamide or ethylene diacrylate as the cross-linking component.
  • How is polyacrylamide made?
  • Derivatives of polyacrylamide are typically made through partial hydrolysis of the amide bond linkages within the gel or through a process of transamidination carried out at elevated temperatures using amine-containing spacer arms or ligands.
  • How are polyacrylamide gels made?
  • Polyacrylamide gels are made by chemical polymerization of a mixture of acrylamide and bisacrylamide (a cross-linker) in the presence of a catalyst and an initiator of the polymerization reaction. The porosity of the gel is determined by the relative concentration of acrylamide to cross-linker and by the total percentage of monomers.
  • What is a polyacrylamide gel?
  • Polyacrylamide gels are three-dimensional networks of acrylamide reacted with the bifunctional reagent N,N '-methylene-bis-acrylamide (abbreviated as Bis) via a free-radical initiated vinyl polymerization mechanism. The pore size of the gel is very reproducible and is directly related to the ratio of acrylamide to Bis.